《植物生理学报》 2015, 51(2): 183-188

不同海拔下烤烟碳氮代谢相关酶基因的表达差异分析

刘炳清¹, 许嘉阳^1,2, 黄化刚³, 杨永霞¹, 杨双剑³, 连培康¹, 许自成^1,*

¹河南农业大学烟草学院, 郑州450002; ²华中农业大学资源与环境学院, 武汉430070; ³贵州省烟草公司毕节市公司, 贵州毕节551700

通信作者：许自成；E-mail: zcxu@sohu.com；Tel: 0371-63555916

摘　要：

为探讨贵州乌蒙烟区不同海拔的烤烟特色形成的分子机理, 本文利用实时荧光定量PCR技术对的烤烟碳氮代谢相关酶基因的表达进行检测。结果表明, 液泡转化酶(VIN)基因在烤烟移栽后50 d的中海拔表达较强, 移栽后60 d高海拔表达比较活跃, 烤烟成熟期低海拔表达略强。蔗糖合成酶(SS)和蔗糖磷酸合成酶(SPS)基因在烤烟旺长期高海拔表达较强, 烤烟成熟期低海拔表达较强, 中海拔表达较弱。在4个取样时期, 海拔越高, 蔗糖磷酸化酶(SPP)基因表达越强, 高海拔地区烟叶的颗粒结合型淀粉合成酶(GBSSI)基因表达均最强。淀粉分支酶(SBE)基因在烤烟移栽后50、60和70 d高海拔下表达较强, 在移栽后80 d低海拔下表达相对较强。从同一时期不同海拔的结果分析, 烤烟硝酸还原酶(NR)基因表达强度随海拔降低而逐渐增强。谷氨酰胺合成酶(GS1-3和GS1-5)基因表达在4个取样时期表现为低海拔强于中、高海拔的。

关键词：烤烟; 海拔; 碳氮代谢; 基因表达

收稿：2014-10-30   修定：2014-12-29

资助：中国烟草总公司贵州省公司重点科技攻关项目(GY2012-06)。

Analysis of Differential Gene Expression of the Related Enzymes in Carbon and Nitrogen Metabolism of Flue-Cured Tobacco at Different Altitudes

LIU Bing-Qing¹, XU Jia-Yang^1,2, HUANG Hua-Gang³, YANG Yong-Xia¹, YANG Shuang-Jian³, LIAN Pei-Kang¹, XU Zi-Cheng^1,*

¹College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, China; ²College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China; ³Bijie Branch of Guizhou Provincial Tobacco Company, Bijie, Guizhou 551700, China

Corresponding author: XU Zi-Cheng; E-mail: zcxu@sohu.com; Tel: 0371-63555916

Abstract:

In order to study the molecular mechanism of characteristics of flue-cured tobacco at different altitudes in Wumeng tobacco-growing areas of Guizhou province, the real time quantitative PCR was used to detect gene expression of the related enzymes in carbon and nitrogen metabolism of flue-cured tobacco. The results indicated that during vigorous growth stage vacuolar invertase (VIN) gene of flue-cured tobaccos in middle and high altitude areas was expressed more actively, and during maturing period VIN gene in low altitude areas was expressed slightly strongly. And Sucrose synthase (SS) and sucrose phosphate synthase (SPS) genes in high altitude areas were expressed more strongly during vigorous growth stage, but were expressed relatively strongly in low altitude areas and more weakly in middle altitude areas during maturing period. During the four sampling periods the higher the altitudes, the more strongly sucrose-6-phosphate phosphatase (SPP) gene was expressed, granule-bound starch synthase I (GBSSI) gene in high altitude areas was expressed most strongly. SBE gene in high altitude areas was expressed more strongly during vigorous growth stage, but relatively strongly in low altitude areas after transplanting 80 days. Based on the analysis of the different altitudes during the same period, with the altitudes lowering gradually, nitrate reductase (NR) gene was expressed more strongly. During the four sampling periods glutamine synthase (GS1-3 and GS1-5) genes in low altitude areas were expressed more strongly than those in middle and high altitude areas.

Key words: flue-cured tobacco (Nicotiana tabacum); altitudes; carbon and nitrogen metabolism; gene expression